After major blood loss of 62% of the circulating blood volume, the animals cannot maintain their blood pressure and expire if fluid resuscitation is not provided (8). When a small volume of E2, and not vehicle, was administered after 62% blood loss, the animals not only demonstrated an acute survival during prolonged hypotension (3 hr), but also prolonged survival after fluid resuscitation.
Yes, we can drain 2/3rds of a rat's blood, shoot it full of estrogen, keep it alive for three hours, and then bring it back to life. As they say, "It's alive! Mwahahahaha!"